Molecular Plant Breeding
Mohammad Saleh Shahvardi; Mohammad mohsenzadeh; Habibollah Habibollah Samizadeh Lahiji
Abstract
This experiment was conducted in order to assess genetic variation and determination of the best genetic structure and the grouping of safflower genotypes using ISSR and retrotransposon markers and different agricultural traits. In this study, 28 safflower genotypes were evaluated using 7 ISSR markers, ...
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This experiment was conducted in order to assess genetic variation and determination of the best genetic structure and the grouping of safflower genotypes using ISSR and retrotransposon markers and different agricultural traits. In this study, 28 safflower genotypes were evaluated using 7 ISSR markers, 3 retrotransposon markers and 12 combined ISSR and retrotransposon markers, as well as eight different morphological traits with different statistical methods. From 22 primers, 117 polymorphic bands were created. The primers, primer-of UBC-827 and TOS-1 with 13 band maximum number of bands, and primer UBC-811 and UBC-822 in combination with 5-band TOS-1 with the lowest band for ISSR primers and Retrotransposons. The average percent polymorphism obtained in this study for markers ISSR, Retrotransposons and primer combinations was from 38.46 to 88.88 percent ISSR and Retrotransposons is variable and the mean percentage of polymorphic for this is equal to 62. High standards of straw gene diversity, Shannon index, the PIC and the number of effective allele primer UBC-810, UBC-811 and primer combination TOS-2 + HB-12, TOS-1 + HB12 and TOS-1+ UBC822 show performance Primers on the assessment of genetic diversity in this article. The morphological traits of 28 genotypes by UPGMA and Euclidean distance criteria were divided into 4 groups. Grouping results of cluster analysis by linear discriminant analysis using Fisher's focal 82.1 percent for morphological traits were confirmed. Principal component analysis showed that the main vectors of the first and second respectively are 8.22 and 6.84 and the 10 first components validated 63.47 percent of total variance. Mantel test the relationship between molecular and morphological data matrix equivalent 0.214 that show little correlation between the two data. Overall, the results showed that there is a considerable genetic variation in safflower germplasm that can be used to select parents and desirable genotypes in safflower breeding programs.
Bioinformatics
Mohammad Mahdi Taghvaei; Habibollah Habibollah Samizadeh Lahiji; Mohammad Reza Bakhtiarizadeh; Mohammad Mohsenzadeh Golafazani
Abstract
Rapeseed (Brassica napus) is one of the most important oil plants in the world. Not only it has rich sources of fatty acids, but also contains protein. Cold stress is one of the growth limiting factors for this plant, which causes massive molecular changes inside the plant. In this research miRNAs, bna-miR164a, ...
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Rapeseed (Brassica napus) is one of the most important oil plants in the world. Not only it has rich sources of fatty acids, but also contains protein. Cold stress is one of the growth limiting factors for this plant, which causes massive molecular changes inside the plant. In this research miRNAs, bna-miR164a, bra-miR5712, bra-miR5717, bna-miR6029, bna-miR6035, bna-N_miR2, bna-N_miR16 that previously had been studied separately in cold and fatty acid biosynthesis, were selected for investigation of the relationship between microRNAs, Cold stress, Fatty Acids and Lipids. Afterwards, bioinformatics prediction of target genes, investigation of gene ontology and their functional pathway and mapping of protein interactions network were performed. As a result, it was discovered that miRNAs which are effective in cold stress, beside regulation of target genes such as SCRM2, HOS1, STZ, GLP4, PP2CA, which are responsible for cold response and thermal stimulus-response, also have regulatory roles on other target genes such as KAS2, FAD8, FAD4, AAD3, FAD7, FAD2 which are involved in biological processes such as unsaturated fatty acid biosynthesis, unsaturated fatty acid metabolism, fatty acid biosynthesis, fatty acid metabolism, cellular lipid catabolism and lipid metabolism, lipid biosynthesis, cellular lipid metabolism. Evaluation of Protein-Protein Interactions revealed that there was a relation between the target genes involved in fatty acid biosynthesis and the target genes involved in cold stress. The next step for subsequent research is to investigate the expression of these miRNAs and their target genes during cold stress and further experimental verifications to prove the regulatory relationship between them.
Biotic and Abiotic stress
Maryam Pasandide arjmand; Habibollah Samizadeh Lahiji; Mohammad Mohsenzadeh Golfazani
Volume 7, Issue 17 , July 2017, , Pages 31-42
Abstract
Drought is one of the most devastating environmental stresses that adversely affect plant metabolic processes. Many plant genes such as photorespiration ones are involve in response to drought stress. In the present study, the effects of drought stress on the expression level of two peroxisomal (Hydroxy ...
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Drought is one of the most devastating environmental stresses that adversely affect plant metabolic processes. Many plant genes such as photorespiration ones are involve in response to drought stress. In the present study, the effects of drought stress on the expression level of two peroxisomal (Hydroxy pyruvate reductase (HPR1) and Glycolate oxidase (GO)) and two mitochondrial (Serine hydroxy methyl transferase (SHMT) and Glycine decarboxylase (GDC)) genes were studied in susceptible (Hayola308) and tolerant (SLM046) genotypes of canola (Brassica napus) under stress (irrigation cut at flowering stage) and non-stress conditions. The result of real time-PCR showed that in Hyola308 genotype the expression level of GO gene at 48, 72 and 96 hours after stress was higher than SLM046 genotype. In Hyola308 genotype, the highest expression level of GDC gene observed at 48 hours of stress and then decreased. The highest relative expression level of SHMT gene in both Hyola308 and SLM046 genotypes detected at 24 hours after stress and then in SLM046 genotype, its level decreased at 48 hours after stress, while in Hyola308 genotype, its expression declined over the time of exposure to stress. SLM046 genotype showed highest amount of HPR1 expression level at 48 hours after stress. It seems that the expression of photorespiration genes in SLM046 genotype increased at the initial times of exposure to stress and with continue the stress, it showed more adaptation to stress and control the photorespiration unlike Hyola308.
